Journal: Emerging Infectious Diseases

Article Title: Candida auris Discovery through Community Wastewater Surveillance during Healthcare Outbreak, Nevada, USA, 2022

doi: 10.3201/eid2902.221523

Figure Lengend Snippet: Culture-based isolation of Candida auris from wastewater, southern Nevada, USA, 2022, showing the effects of incubation temperature and fluconazole selection on competing organisms. A, B) Incubating the enrichment broth at 42°C (B) instead of 40°C (A) mitigated growth of competing filamentous fungi present in wastewater. C, D) Adding fluconazole to the salt Sabouraud dulcitol broth (SSDB) enrichment broth was essential for recovering C. auris from the southern Nevada wastewater samples. We plated 100 μL aliquots of inoculated enrichment broths incubated at 42°C after 2 days on HardyCHROM Candida medium (Hardy Diagnostics, https://hardydiagnostics.com ) or BBL CHROMagar Candida medium (Becton, Dickinson, and Co., https://www.bd.com ). Inoculation in SSDB without fluconazole resulted in overgrowth of an unidentified competing yeast (C), whereas inoculation in SSDB with fluconazole enabled isolation of C. auris colonies (D).

Article Snippet: We used 100 μL of this suspension to inoculate 2 mL of S 2 Media salt Sabouraud dulcitol broth (SSDB; Thomas Scientific, https://www.thomassci.com ) ( ) containing fluconazole (SSDBF) at a final concentration of 8 μg/mL.

Techniques: Isolation, Incubation, Selection

Journal: BMC Molecular Biology

Article Title: Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans -splicing group I introns

doi: 10.1186/1471-2199-11-84

Figure Lengend Snippet: Assessment of αDENV-GrpI bicistronic constructs activities using luciferase assays . A) Drosophila S2 cells were co-transfected with 3 μg of each αDENV-GrpI, 1 μg double-stranded DENV-2 target (+) or control plasmid (-), and 0.5 μg of IRL expression plasmids. Following cell lysis, samples were processed and assayed for luciferase activity (see Methods). The error bars represent standard deviations of three independent experiments. B) Aag2 cells co-transfected with αDENV-GrpI and pA5c-IRL expression plasmids were challenged with DENV-2 NGC at an MOI of 0.01 24 h post transfection (+). Control cells were transfected with an empty pUC57 plasmid (-) and challenged with virus in the same manner. The error bars represent standard deviations of three independent experiments. B = BQCV IRES. D = DCV IRES. RL = Renilla luciferase. RLU = Relative Luciferase Units.

Article Snippet: Aag2 cells were plated into 9.6 cm 2 well in minimal S2 media (Lonza) at a density of 1.0 × 10 6 cells/well.

Techniques: Construct, Luciferase, Transfection, Control, Plasmid Preparation, Expressing, Lysis, Activity Assay, Virus